By CRISPR/cas9 and non-HDR, the periodicity of stable F2 knockout larvae is 7-10 month.
By CRISPR/cas9 and HR, the periodicity of stable F2 knockout larvae is 7-12 month.
By CRISPR/cas9 and ssODN-mediated HDR, the periodicity of stable F2 knockout larvae is 7-10.
Knockout lines are constructed by CRISPR/Cas9, the periodicity of stable F2 knockout larvae is 7-8.
Transgene by tol2 transposase, the periodicity of stable F2 transgenic larvae is 7-8 months.